2014-03-13

This book deals with Production of cyclodextrin glucanotransferase (CGTase) from Bacillus subtilis isolated from agriculture field soil samples. Initial screening

Purpose γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. However, the low specificity and activity of soluble γ-CGTase increase the production cost of γ-CD, thereby limiting its applications. Therefore, the present study aimed 2009-07-08 · Activity characteristics and kinetic aspects of a cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans DF 9R were studied. A mixture of α-, β- and γ-cyclodextrins (CDs), glucose, maltose and negligible amounts of longer linear dextrins were produced from gelatinized amylose, amylopectin and starch from different sources. Cyclodextrin glucanotransferase (CGTase) is an important industrial enzyme which is used to produce cyclodextrins. CGTase genes from more than 30 bacteria have been isolated and several of the enzymes have been identified and biochemically characterized. For a better understanding of the reaction mechanism and function of CGTase, the enzyme has been analyzed at gene level and protein level cgtase.

The enzyme is extremely heat stable and has a temperature optimum of 90-95°C at pH 6.0. It is active over a broad pH range, and exhibits more than 80% activity from pH 5.0-6.7. CGTase are known to catalyze four different transferase reactions: cyclization, coupling, disproportionation, and hydrolysis. CGTase are classified in the α-amylase family, which includes α-amylase, isoamylase, pullulanase, amylopullulanase, neopullulanase, and the branching enzyme (28, 34, 46). The various CGTase activities were measured by incubating appropriately diluted enzyme (0.1–0.2 units of specific activity for the specific reactions) at 50 °C with substrate solutions in 10 m m sodium citrate (pH 6.0). Cyclodextrin glycosyltranferase (CGTase EC 2.4.1.19) is a bacterial enzyme that converts starch and other 1,4-linked α-glucans to cyclodextrins (closed-ring structures composed mainly of 6, 7 and 8 glucosyl units, named α, β and γ-cyclodextrin, respectively) (French, 1957; Thoma and Stewart, 1965). Cyclodextrins have the ability to form inclu- CGTase (EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch or cyclo-dextrine as donor.

First starch is liquified either by heat treatment or using α-amylase, then CGTase is added for the enzymatic conversion. Immobilisation of CGTase for continuous production of long-carbohydrate-chain alkyl glycosides Control of product distribution by flow rate adjustment.

First starch is liquified either by heat treatment or using α-amylase, then CGTase is added for the enzymatic conversion. CGTases produce mixtures of cyclodextrins, thus the product of the conversion results in a mixture of the three main types of cyclic molecules, in ratios that are strictly dependent on the enzyme used: each CGTase has its

Stipendiat. 24B Korpraditskul,.

Cyclodextrin glucanotransferase [CGTase, E.C.2.4.1.19] is an extracellular enzyme, which catalyzes the formation of α−, β−, γ− CDs from starch.

CGTases mainly perform three different reactions in addition to hydrolysis, namely cyclization, disproportionation and coupling. cgtase - Cyclodextrin glycosyltransferase - Anaerobranca gottschalkii - cgtase gene & protein UniProtKB - Q5ZEQ7 (Q5ZEQ7_9FIRM) γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are extensively used in agriculture, chemicals, cosmetics, foods and pharmaceuticals . Actually, CGTase has been discovered in a great number of Bacillus, Thermoanaerobacter, Brevibacterium and Thermoanaerobacterium [8,9,10].

The CGTase gene from the hyperthermophilic archaea Archaeoglobus fulgidus (AfCGTase) has 34 % rare codons as compared to Escherichia coli genes. Het product wordt verkregen door de inwerking van het enzym cycloglycosyltransferase (CGTase) uit Bacillus circulans, Paenibacillus macerans of recombinant Glycosyltransferas (CGTase). Cyclodextrin (CD) and. Paenibacillus Sp A11, CLIPtechnique.
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Cyclodextrin (CD) and. Paenibacillus Sp A11, CLIPtechnique. Stipendiat. 24B Korpraditskul,.

Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i. Ca 2+ ARBA annotation.
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CGTase enzymes immobilized on various supports such as porous glass beads or glyoxyl-agarose have been previously used to achieve this objective. Nevertheless, the attachment of biocatalysts on conventional supports is associated with numerous drawbacks, including enzyme leaching prominent in physical adsorption, reduced activity as a result of chemisorption, and increased mass transfer

The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related α-1,4-glucans. CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. CGTases differ in the amount and types of CDs produced.

2016-04-15

Oct 25, 2018 Abstract Cyclodextrin glucanotransferases (CGTases) synthesize cyclic oligosaccharides (cyclodextrins, CD) from starch. A CGTase from The results suggest that the overexpression level of recombinant CGTase excreted into the culture medium using the recombinant Escherichia coli could be In enzymology, a cyclomaltodextrin glucanotransferase (also cyclodextrin glycosyl transferase or CGTase for short) (EC 2.4.1.19) is an enzyme that catalyzes the 17 matches Synonym: FTase Inhibitor I - Calbiochem · Empirical Formula (Hill Notation): C22 H38N4O3S Molecular Weight: 470.69. Nov 12, 2019 ABSTRACT. Cyclodextrin glycosyltransferase. (CGTase) catalyzes the formation of cyclodextrins from starch. Among the CGTases with known Sep 10, 2017 Interestingly, the immobilized cell on the non-treated hollow fiber membrane showed up to 15% increment of CGTase excretion with 55% CGTase the starch slurry is gelatinized by cyclized by CGTase to produce CD Cyclodextrin glycosyl transferase (CGTase) fermentation was carried out Abstract.

In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - CGTase is an important industrial enzyme that can transfer starch to glycosyl groups to form cyclodextrin (Li et al., 2014c; Qi et al., 2007). CGTase has been found in many bacteria, including Bacillus spp. (Chen et al., 2018; Gimenez et al., 2019; Yap et al., 2010) and Paenibacillus spp. (Castillo et al., 2018).